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Biochemistry

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JoVE Journal Biochemistry
LC-MS / MS分析を用いた核補因子と相互作用するタンパク質のハイスループット同定のためのタンパク質調製方法
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Journal (Biochemistry)
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Protein Isolation from Sipunculus nudus (Video) | JoVE
Journal (Biochemistry)
Protein Isolation from Sipunculus nudus (Video) | JoVE
 
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LC-MS / MS分析を用いた核補因子と相互作用するタンパク質のハイスループット同定のためのタンパク質調製方法

Article DOI: 10.3791/55077-v • 05:43 min • January 24th, 2017
January 24th, 2017 •
*1, *2, 3, 1, 3,4,5
1Graduate School of Frontier Biosciences, Osaka University, 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences, Doshisha University
* These authors contributed equally

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요약
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我々は、LC-MS / MSシステムを使用して共調節相互作用タンパク質の精製のための方法を確立しました。

Tags

生化学号119、タンパク質相互作用、LC-MS / MS、タンパク質精製、免疫沈降、核内受容体補因子、転写、クロマチンリモデリング因子、タンパク質複合体
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