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JoVE Journal Biochemistry
Måling af G-proteinkoblet receptorreceptor via radio-mærket GTP-binding
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Måling af G-proteinkoblet receptorreceptor via radio-mærket GTP-binding

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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Guanosintriphosphat (GTP) -binding er en af ​​de tidligste begivenheder i G-protein-koblet receptor (GPCR) -aktivering. Denne protokol beskriver, hvordan man farmakologisk karakteriserer specifikke GPCR-ligandinteraktioner ved at overvåge bindingen af ​​den radiomærkede GTP-analog, [35S] guanosin-5'-O- (3-thio) triphosphat ([35S] GTPγS), i Svar på en ligand af interesse.

Tags

Biochemistry Cellular fractionation membrane preparation GPCR agonist antagonist GTPγS binding pharmacology μ-opioid receptor
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