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Developmental Biology

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JoVE Journal Developmental Biology
Bearbetning av mänskliga hjärtvävnad mot extracellulära Matrix självmonterande Hydrogel för In Vitro och In Vivo applikationer
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Bearbetning av mänskliga hjärtvävnad mot extracellulära Matrix självmonterande Hydrogel för In Vitro och In Vivo applikationer

Article DOI: 10.3791/56419-v • 08:41 min • December 4th, 2017
December 4th, 2017 •
1,2, 1,2, 2, 2, 1,2, 2,3,4,5, 2,3,4
1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

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Det här protokollet beskriver den kompletta decellularization av mänskliga hjärtmuskeln samtidigt bevara dess extracellulär matrix komponenter. Vidare bearbetning av extracellulärmatrix resulterar i produktion av mikropartiklar och en cytoprotektiva själv montering hydrogel.

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Utvecklingsbiologi fråga 130 extracellulär matrix mänskliga hjärtmuskeln hydrogel decellularization cytoprotection mikropartiklar
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