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Journal / Developmental Biology / 用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像…
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Developmental Biology

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用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像

Article DOI: 10.3791/57059-v • 11:19 min • March 20th, 2018
March 20th, 2018 •
1,2, 3, 2,4, 2,4, 5, 6, 2,4, 1,2,3
1Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility, Universität Heidelberg

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本协议以扫描电镜 (SEM) 为目标, 以纳米尺度分辨率成像组织中的特定细胞为靶点。从树脂嵌入的生物材料中, 大量的系列部分首先在光学显微镜中被成像, 以识别目标, 然后以分层的方式在 SEM 中。

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发育生物学 问题 133 阵列层析成像 大体积电子显微镜 扫描电镜 荧光光镜 相关的光和电子显微镜 克莱姆 分级成像 自动成像 靶向
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