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JoVE Journal
Chemistry

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JoVE Journal Chemistry

Microfluidic Chips voor In Situ Crystal röntgendiffractie en In Situ Dynamische lichtverstrooiing voor seriële kristallografie

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Microfluidic Chips voor In Situ Crystal röntgendiffractie en In Situ Dynamische lichtverstrooiing voor seriële kristallografie

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018

April 24th, 2018 •

Yannig Gicquel*^1,2, Robin Schubert*^3,4,5, Svetlana Kapis³, Gleb Bourenkov⁶, Thomas Schneider⁶, Markus Perbandt^3,4, Christian Betzel^3,4,5, Henry N. Chapman^1,2,4, Michael Heymann^1,7

¹Center for Free Electron Laser Science, DESY, ²Department of Physics, University of Hamburg, ³Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, ⁴The Hamburg Center for Ultrafast Imaging, University of Hamburg, ⁵Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), ⁶European Molecular Biology Laboratory, EMBL c/o DESY, ⁷Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry

* These authors contributed equally

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Dit protocol beschrijft in detail hoe te fabriceren en te exploiteren van microfluidic apparaten voor röntgendiffractie gegevensverzameling bij kamertemperatuur. Bovendien, het beschrijft hoe eiwitten kristallisatie controleren door Dynamische lichtverstrooiing en hoe om te verwerken en analyseren diffractie gegevens verkregen.

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Chemie kwestie 134 in situ röntgendiffractie vaste doelstelling seriële milliseconde kristallografie microfluidics eiwit kristallisatie in situ dynamische licht verstrooiing

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