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Developmental Biology

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Identificatie van de skeletspieren satelliet cellen met immunofluorescentie met Pax7 en Laminin antilichamen

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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De nauwkeurige identificatie van satelliet cellen is essentieel voor de studie van hun functies onder verschillende fysiologische en pathologische omstandigheden. Dit artikel presenteert een protocol ter identificatie van de satelliet cellen op volwassen skeletspieren secties door immunofluorescentie gebaseerde kleuring.

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Ontwikkelingsbiologie kwestie 134 skeletspieren satelliet cellen Pax7 immunofluorescentie stamcel basale lamina
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