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Journal / Neuroscience / 高频刺激激活小鼠齿状回的侵袭方法
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高频刺激激活小鼠齿状回的侵袭方法
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高频刺激激活小鼠齿状回的侵袭方法

Article DOI: 10.3791/57857-v • 12:26 min • June 2nd, 2018
June 2nd, 2018 •
1, 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

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该协议说明如何在小鼠中建立可靠的痉挛方法。整个海马齿状回的神经元由痉挛直接和间接的在体内电刺激。神经活动和分子信号由c-fos和 Notch1 免疫荧光染色分别检查;bromodeoxyuridine 标记法对神经发生进行量化。

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神经科学 问题 136 高频 刺激 齿状回 神经元活动 神经发生 BrdU 标记
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