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Journal / Immunology and Infection / Identificatie van bemiddelaars/Mediators van…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Identificatie van bemiddelaars/Mediators van T-cel Receptor signalering via de Screening van chemische remmer bibliotheken
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Identificatie van bemiddelaars/Mediators van T-cel Receptor signalering via de Screening van chemische remmer bibliotheken

Article DOI: 10.3791/58946-v • 08:49 min • January 22nd, 2019
January 22nd, 2019 •
*1, *2,3, *1, 1, 1,4
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven
* These authors contributed equally

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Deze paper gebruikt een stroom cytometry-gebaseerde assay naar scherm bibliotheken van chemische inhibitoren voor de identificatie van remmers en de bijbehorende doelen die van invloed zijn T-cel receptor signalering. De hier beschreven methoden kunnen ook worden uitgebreid voor high-throughput screening.

Tags

Immunologie en infecties probleem 143 activering van Caspase-3 kinase-remmers bibliotheek screening TCR signalering T-cel activatie thymocyte selectie
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