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Chromatin Immunoprecipitation Assay mit Mikrokokken-Nukleasen in Säugetierzellen

Article DOI: 10.3791/59375-v • 11:42 min • May 10th, 2019
May 10th, 2019 •
1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

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Chromatin-Immunpräzipitation (ChIP) ist ein leistungsfähiges Werkzeug zum Verständnis der molekularen Mechanismen der Genregulation. Das Verfahren birgt jedoch Schwierigkeiten bei der Erlangung reproduzierbarer Chromatinfragmentierung durch mechanisches Scheren. Hier bieten wir ein verbessertes Protokoll für einen ChIP-Assay mit enzymatischer Verdauung.

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Genetik Ausgabe 147 Genregulation Histonmodifikation Chromatin-Immunpräzipitation Mikrokokken-Nuklease RNA-Polymerase II trimethyliertes Histon H3-Lysin 4
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