Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Test standardisé et évolutif pour étudier…
JoVE Journal
Bioengineering

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Bioengineering
Test standardisé et évolutif pour étudier perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions
Journal (Bioengineering)
Semi-Automated Mechanical Dissociation of Mouse Tissues to Generate Single-Cell Suspensions
Zebrafish Larva Preparation, Mounting, and Light-Sheet Imaging for Cardiac Development Studies
Journal (Bioengineering)
Zebrafish Larva Preparation, Mounting, and Light-Sheet Imaging for Cardiac Development Studies
 
Click here for the English version

Test standardisé et évolutif pour étudier perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Cette méthode décrit la culture des cellules endothéliales dérivées de l'iPSC comme 40 microvaisseaux 3D perfusés dans une plate-forme microfluidique normalisée. Cette plate-forme permet l'étude de la germination angiogénique à gradient en 3D, y compris l'anastomose et la stabilisation des germes angiogéniques d'une manière évolutive et à haut débit.

Tags

Bioingénierie Numéro 153 Culture cellulaire 3D microfluidique in vitro cellules souches pluripotentes induites par l'homme cellules endothéliales angiogenèse gradients dépistage à haut débit développement d'analyse
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter