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Enkelt celle mikro-aspiration som en alternativ strategi for fluorescens-aktiverede celle sortering for Giant virus blanding adskillelse

Article DOI: 10.3791/60148-v • 09:50 min • October 27th, 2019
October 27th, 2019 •
1, 2, 2, 1, 1,2, 2
1Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique - Hôpitaux de Marseille (AP-HM)

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Her beskriver vi en enkelt celle mikro-aspiration metode til adskillelse af inficerede amoebae. For at adskille virale subpopulationer i Vermamoeba vermiformis inficeret af Faustovira og ukendte gigantiske vira, vi udviklede protokollen nedenfor og viste sin evne til at adskille to lav-overflod roman Giant vira.

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Biologi enkelt celle mikro-aspiration kloning Giant vira Faustovirus co-kultur Vermamoeba vermiform flow cytometri FACS
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