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Time-lapse Live Imaging og kvantificering af hurtig dendritiske gren dynamik i at udvikle Drosophila neuroner

Article DOI: 10.3791/60287-v • 08:23 min • September 25th, 2019
September 25th, 2019 •
1, 1, 1, 1, 1, 1
1National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Her beskriver vi den metode, vi anvendte til at afbilde meget motile sædceller dendritiske filopodia i en levende forberedelse af Drosophila larve hjernen, og den protokol, vi udviklede til at kvantificere tidsforskudt 3D imaging datasæt for kvantitative vurderinger af dendrit dynamik i udviklingen af neuroner.

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Neurovidenskab dendrit udvikling dendritiske filopodia gren dynamik Drosophila ventrale laterale neuroner time-lapse Imaging
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