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Neuroscience

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Time-lapse konfokal billeddannelse af migrerende neuroner i organotypisk skive kultur af embryonale mus hjernen ved hjælp af
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Time-lapse konfokal billeddannelse af migrerende neuroner i organotypisk skive kultur af embryonale mus hjernen ved hjælp af

Article DOI: 10.3791/55886-v • 13:33 min • July 25th, 2017
July 25th, 2017 •
1, 1, 1, 1
1Institute of Molecular and Cellular Anatomy, Ulm University

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Denne protokol indeholder instruktioner til direkte observation af radialt migrerende kortikale neuroner. I utero- elektroporering, organotypisk skivekultur og time-lapse konfokal billeddannelse kombineres for direkte og dynamisk undersøgelse af virkningerne af overekspression eller nedregulering af gener af interesse for migrerende neuroner og for at analysere deres differentiering under udvikling.

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Neurobiologi udgave 125, organotypisk skivekultur time-lapse-billeddannelse radial migration neocortex musemodel neuronal migrationsforstyrrelser
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