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Bioengineering

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Fibroblasten-abgeleitetes Human Engineered Connective Tissue für Screening-Anwendungen

Article DOI: 10.3791/62700-v • 09:50 min • August 20th, 2021
August 20th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2,3,4,5, 1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)

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Hier wird ein Protokoll zur Erzeugung von künstlichem Bindegewebe für eine Parallelkultur von 48 Geweben in einer Multi-Well-Platte mit Doppelpolen vorgestellt, die für mechanistische Studien, Krankheitsmodellierung und Screening-Anwendungen geeignet ist. Das Protokoll ist kompatibel mit Fibroblasten aus verschiedenen Organen und Spezies und wird hier mit menschlichen primären kardialen Fibroblasten veranschaulicht.

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Bioengineering Ausgabe 174 Tissue Engineering Herzfibroblast Kollagenmatrix Gewebesteifigkeit Screening
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