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Journal / Biology / Isolering av humane nøytrofiler fra fullblod og…
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Isolering av humane nøytrofiler fra fullblod og buffyfrakker

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

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Denne protokollen beskriver en metode for isolering av nøytrofiler fra fullblod, buffy strøk eller leukaferesemembraner, oppnå godt utbytte, høy renhet og minimal celleaktivering. Vi bruker gradient rensing, rød blodcelle (RBC) sedimentering, og RBC lysis for å oppnå en høy kvalitet / renhet nøytrofil forberedelse.

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Biologi Utgave 175 Nøytrofil gradient dextran RBC lysis fullblod buffy frakk
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