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Journal / Biochemistry / Tijd-opgeloste Förster Resonantie Energie…
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Biochemistry

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JoVE Journal Biochemistry
Tijd-opgeloste Förster Resonantie Energie Transfer Assays voor meting van endogene gefosforyleerde STAT-eiwitten in menselijke cellen
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Tijd-opgeloste Förster Resonantie Energie Transfer Assays voor meting van endogene gefosforyleerde STAT-eiwitten in menselijke cellen

Article DOI: 10.3791/62915-v • 07:12 min • September 9th, 2021
September 9th, 2021 •
1, 1, 1
1BioAuxilium Research

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Tijd-opgeloste Förster resonantie energieoverdracht cel-gebaseerde assay protocollen worden beschreven voor de eenvoudige, specifieke, gevoelige en robuuste kwantificering van endogene gefosforyleerde signaal transducer en activator van transcriptie (STAT) 1/3/4/5/6 eiwitten in cellysaten in een 384-well formaat.

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Biochemie JAK/STAT signalering STAT eiwitten Fosforylering Homogene assays TR-FRET Immunoassay Cell-based assay Kinases Cell signaling High-throughput screening Cytokine signaling
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