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Flowcytometrische analyse van meerdere mitochondriale parameters in door de mens geïnduceerde pluripotente stamcellen en hun neurale en gliaderivaten

Article DOI: 10.3791/63116-v • 06:09 min • November 8th, 2021
November 8th, 2021 •
*1,2, *1,2,3,4, 1,2, 1,2
1Department of Clinical Medicine (K1), University of Bergen, 2Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, 3Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, 4Shandong Key Laboratory of Brain Function Remodeling
* These authors contributed equally

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Deze studie rapporteert een nieuwe benadering om meerdere mitochondriale functionele parameters te meten op basis van flowcytometrie en dubbele kleuring met twee fluorescerende melders of antilichamen om veranderingen in mitochondriaal volume, mitochondriaal membraanpotentiaal, reactief zuurstofsoortniveau, mitochondriale ademhalingsketensamenstelling en mitochondriaal DNA te detecteren.

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Neurowetenschappen Nummer 177
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