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Engineering

이 콘텐츠은(는) 무료 액세스입니다.

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Imaging chimico multiplex basato sulla microscopia a dispersione Raman stimolata a banda larga
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Imaging chimico multiplex basato sulla microscopia a dispersione Raman stimolata a banda larga

Article DOI: 10.3791/63709-v • 09:57 min • July 25th, 2022
July 25th, 2022 •
1, 1, 1, 1, 1, 1, 2, 1,2, 1,2
1Dipartimento di Fisica, Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)

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Presentiamo un protocollo per acquisire immagini chimiche con microscopia a diffusione Raman stimolata a banda larga (SRS). Basato su un microscopio SRS che opera con rilevamento differenziale multicanale-lock-in, il protocollo descrive la preparazione del campione, la regolazione dell'apparato SRS e la chemiometria per districare diversi costituenti di campioni chimicamente eterogenei.

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Engineering Raman scattering stimolazione Raman scattering microscopia non lineare iperspettrale banda larga imaging vibrazionale
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