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Levende billeddannelse af mikrotubulusdynamik i glioblastomceller, der invaderer zebrafiskhjernen

Article DOI: 10.3791/64093-v • 09:29 min • July 29th, 2022
July 29th, 2022 •
1, 1, 1
1Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

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Vi rapporterer en teknik, der tillader levende billeddannelse af mikrotubulusdynamik i glioblastom (GBM) celler, der invaderer et hvirveldyrs hjernevæv. Kobling af den ortopiske injektion af fluorescerende mærkede GBM-celler til en gennemsigtig zebrafiskhjerne med intravital billeddannelse med høj opløsning muliggør måling af cytoskeletdynamik under in situ kræftinvasion.

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Biologi udgave 185 Ortopisk xenograft PDX Zebrafisk Danio rerio Glioblastom Subcellulær intravital billeddannelse 5D billedanalyse
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