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Journal / Biology / Mitofagie visualiseren met fluorescerende…
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Mitofagie visualiseren met fluorescerende kleurstoffen voor mitochondriën en lysosoom

Article DOI: 10.3791/64647-v • 07:56 min • November 30th, 2022
November 30th, 2022 •
1,2, 1, 3, 1, 1, 1,2
1Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, 2Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 3Department of Pharmacy, Shanghai East Hospital, Tongji University

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Mitofagie is het primaire mechanisme van mitochondriale kwaliteitscontrole. De evaluatie van mitofagie in vivo wordt echter belemmerd door het gebrek aan betrouwbare kwantitatieve testen. Hier wordt een protocol gepresenteerd voor de observatie van mitofagie in levende cellen met behulp van een celpermeant groen-fluorescerende mitochondriënkleurstof en een rood-fluorescerende lysosoomkleurstof.

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Biologie Nummer 189
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