In Vitro Exposure System
In Vitro Exposure System - Related Videos
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
07:01
A New Portable In Vitro Exposure Cassette for Aerosol Sampling
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Cited by 4 •
2019
Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.
08:06
A Standardized In Vitro Procedure For Evaluating Surface Microhardness Changes In Nanohybrid Resin Composites After Whey Protein Beverage Exposure
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2026
This protocol describes a standardized in vitro method for evaluating the effects of whey protein beverages on resin composite microhardness. The approach enables controlled assessment of dietary exposure using cyclic immersion and Vickers testing, providing a reproducible model for investigating the interaction between nutritional beverages and restorative biomaterials.
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
07:05
In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface
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Cited by 1 •
2025
We establish a standardized in vitro model for studying cannabis vapor exposure at the air-liquid interface. This model provides a systematic approach to examine the impacts of vaporized cannabis, addressing the growing interest in cannabis vaporizers as an alternative to smoking.
FAQs
Dose and exposure duration define how much stimulus reaches the biological model and how long the model experiences it. Interpreting these variables together helps researchers distinguish responses associated with the amount of an agent from those associated with prolonged exposure. Keeping both factors defined supports comparisons across experiments and makes biological outcomes easier to relate to engineered design choices.
The exposure interface provides a controlled point of contact between the test agent and the biological model. Its design affects how consistently the stimulus reaches the model, while temperature and surrounding culture conditions help preserve the intended experimental environment. Maintaining this interface is important for measuring cellular signaling, toxicity, biocompatibility, or material-related responses without losing control of key variables.
Reproducibility depends on defining the stimulus, dose, exposure duration, delivery route, temperature, and surrounding culture conditions before measurements begin. Changes in any of these factors can alter the biological response or make results difficult to compare. A carefully specified exposure design therefore helps connect observed effects to the tested condition rather than to uncontrolled differences between experiments.
A basic workflow starts by selecting the cells, tissues, construct, organoid, or other engineered model and identifying the substance, environmental condition, or stimulus to test. Researchers then specify dose, duration, delivery route, temperature, and culture conditions, establish the exposure interface, and measure the resulting biological response. This sequence links the applied condition with a defined experimental outcome.
Researchers may choose this approach when they need to evaluate how an engineered material, construct, organoid, or related model responds to a defined test condition. It can support studies of biocompatibility, toxicity, cellular signaling, and material performance. Because the exposure variables are controlled, the system helps assess biological consequences before moving to more complex testing stages.
The resulting measurements can show whether a tested condition is associated with changes in biocompatibility, toxicity, cellular signaling, or material performance. In bioengineering, these outcomes help relate design choices to biological behavior in constructs, organoids, and other in vitro models. The controlled setting also supports more reproducible comparisons among exposure conditions and engineered configurations.