Murine Liver
Murine Liver - Related Videos
03:18
In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein
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2023
This video describes a procedure for mouse liver perfusion through the portal vein using collagenase solution prior to isolating liver cells.
07:27
The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
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Cited by 14 •
2017
Here we describe a common method to induce chronic liver injury in mice by feeding of a choline-deficient and ethionine-supplemented (CDE) diet. We demonstrate health monitoring, liver perfusion, isolation, and preservation. A time course of six weeks can inform about liver injury, pathohistology, fibrosis, inflammatory, and liver progenitor cell responses.
12:36
Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
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Cited by 16 •
2020
This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
07:55
The Influence of Liver Resection on Intrahepatic Tumor Growth
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Cited by 6 •
2016
A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.
FAQs
Lobules organize hepatocytes around sinusoids, the vascular spaces through which blood-borne nutrients, signaling molecules, and compounds pass. This arrangement links local liver cells to circulating influences that can affect tumor initiation and progression. Studying these structural relationships helps researchers connect cellular or molecular changes with the whole-organ environment rather than examining tumor cells in isolation.
The liver processes nutrients, signaling molecules, and compounds delivered through the blood. Consequently, the organ can modify exposures and signals that influence how tumors begin or develop. This feature makes murine liver models useful for examining cancer biology in a physiological setting, where tumor behavior reflects interactions between transformed cells, circulating factors, and liver organization.
These models provide complementary ways to investigate hepatocarcinogenesis and tumor behavior. Genetically engineered mice examine cancer in the context of defined genetic changes, chemically induced models examine tumors arising after exposure to tumor-promoting compounds, and transplantation models support focused analysis of tumor growth or interactions within the liver. The appropriate choice depends on the research question.
The liver microenvironment includes the organized tissue structure, sinusoids, hepatocytes, and signals exchanged with blood-borne factors. These elements can influence tumor initiation, progression, and interactions between cancer cells and surrounding tissue. Murine models allow investigators to study those relationships at the organ level, complementing molecular measurements made within tumor cells.
A study generally selects a model suited to its question, such as a genetically engineered, chemically induced, or transplantation system, and then examines tumor development or behavior in the liver. Researchers can assess hepatocarcinogenesis, tumor–microenvironment interactions, therapeutic response, or metastasis. The model links molecular and cellular observations with whole-organ physiology.
They are useful when investigators need to examine therapeutic response within an intact liver and its surrounding biological environment. In addition to tumor behavior, these models can support analysis of how treatment relates to tumor–microenvironment interactions and whole-organ physiology. Such context may provide information that isolated cellular systems cannot capture on their own.
Murine liver models connect cellular and molecular findings with whole-organ physiology, making them valuable for studying hepatocarcinogenesis, treatment response, and metastasis. However, mouse and human liver biology differ. Researchers must therefore consider those differences when judging translational relevance and avoid assuming that an observation in mice will directly predict human cancer behavior or treatment outcomes.