This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.
Pearen, M. A., Lim, H. K., Gratte, F. D., Fernandez-Rojo, M. A., Nawaratna, S. K., Gobert, G. N., Olynyk, J. K., Tirnitz-Parker, J. E. E., Ramm, G. A. Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology. J. Vis. Exp. (157), e60992, doi:10.3791/60992 (2020).