Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Michael A. Pearen; Hong Kiat Lim; Francis D. Gratte; Manuel A. Fernandez-Rojo; Sujeevi K. Nawaratna; Geoffrey N. Gobert; John K. Olynyk; Janina E. E. Tirnitz-Parker; Grant  A. Ramm

doi:10.3791/60992

Journal

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Medicine

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Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

JoVE Journal
Medicine
Author Produced

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JoVE Journal Medicine

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

15,787 Views

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12:36 min

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March 14, 2020

DOI:

10.3791/60992-v

DOI:

10.3791/60992-v

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12:36 min

March 14, 2020

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15761 Views

Michael A. Pearen*¹, Hong Kiat Lim*¹, Francis D. Gratte^2,3, Manuel A. Fernandez-Rojo^1,4,5, Sujeevi K. Nawaratna⁶, Geoffrey N. Gobert⁷, John K. Olynyk^8,9, Janina E. E. Tirnitz-Parker^2,10, Grant A. Ramm^1,4

¹Hepatic Fibrosis Group,QIMR Berghofer Medical Research Institute ²School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute,Curtin University ³School of Veterinary and Life Sciences,Murdoch University ⁴School of Medicine,The University of Queensland ⁵Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC ⁶School of Medicine,Griffith University ⁷School of Biological Sciences,Queen’s University Belfast ⁸Department of Gastroenterology & Hepatology,Fiona Stanley and Fremantle Hospital Group ⁹School of Medical and Health Sciences,Edith Cowan University ¹⁰Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences,University of Western Australia

Summary

This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.