Mouse Model Metastasis
Mouse Model Metastasis - Related Videos
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
08:11
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
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Cited by 83 •
2016
An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.
05:06
A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
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Cited by 2 •
2015
The metastatic spread of cancer is the major cause of cancer-related deaths. We provide an in-depth description of our survival surgery methodology for establishing a “patient-like” orthotopic syngeneic mouse model system for studying the mechanisms of metastasis in solid organ tumors.
07:43
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
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Cited by 32 •
2017
Brain metastasis has become an urgent unmet medical need as its incidence has increased while therapeutic options have remained palliative. Creating experimental animal models of brain metastasis via intracarotid arterial injection of cancer cells facilitates mechanistic studies of the disease biology and evaluation of novel intervention regimens.
08:49
Models of Bone Metastasis
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Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
FAQs
Spontaneous models begin with tumor cells placed at an organ-specific site, allowing researchers to observe dissemination from a primary tumor. In circulation-based experiments, cells enter the bloodstream directly, so the investigation focuses on later events, including vascular escape and colonization of another tissue. This distinction helps separate early dissemination mechanisms from the processes that establish secondary lesions.
These conditions can shape how metastatic progression unfolds in the mouse model. A defined genetic background, selected tumor microenvironment, or particular immune condition provides a controlled setting for examining factors that regulate spread. Comparing models with different conditions can help researchers determine whether an observed metastatic pattern depends on the cancer cells, their surrounding tissue, or immune context.
Introducing tumor cells into the circulation enables focused study of events that occur after bloodstream entry. Researchers can examine whether cells leave the vasculature and establish lesions in distant tissue, rather than concentrating on escape from the original tumor. These observations clarify which later steps of metastatic progression may be influenced by specific biological factors or treatments.
The experimental route depends on the stage of metastasis being investigated. Researchers may implant tumor cells at a selected organ-specific site to model spread from a primary location, or introduce cells into the circulation to examine downstream events. The chosen approach determines which parts of metastatic progression can be observed and compared within the study.
These models support several cancer research goals, including investigating metastatic biology, identifying factors that regulate progression, and evaluating potential therapeutic targets. They also allow researchers to test treatments designed to limit metastasis. Because the models can incorporate defined biological conditions, experiments can connect a candidate mechanism with changes in dissemination or secondary lesion formation.
Researchers assess how closely experimental findings reflect human disease by considering the model's tumor setting, genetic background, microenvironment, and immune conditions. Results are most informative when the selected conditions address the biological context under investigation. This evaluation helps place observations about metastatic progression, therapeutic targets, or treatment effects within an appropriate cancer research context.