Bfu-e Progenitors
Bfu-e Progenitors - Related Videos
12:55
Purification of Progenitors from Skeletal Muscle
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Cited by 31 •
2011
Method for the enzymatic dissociation, surface labeling and purification by flow cytometry of fibro/adipogenic and myogenic progenitors from murine skeletal muscle.
02:15
Differentiation of Neural Progenitor Cells into Neurons
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2025
The video showcased a cell culture method for differentiating neural progenitor cells into neurons. By incubating cells in a nutrient-rich medium supplemented with growth factors, the progenitor cells matured into neurons with developed axons and dendrites. A stabilizing agent ensured cell viability and protected against oxidative damage throughout the differentiation process.
14:26
Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
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Cited by 38 •
2012
Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth factors.
12:03
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Cited by 18 •
2012
A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.
08:59
Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
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Cited by 13 •
2017
While infiltrating macrophages are continuously recruited to adult tissues from circulating precursors, resident macrophages seed their tissue during development, where they are maintained without further input from progenitors. The progenitors for resident macrophages were recently identified. Here, we present methods for the genetic fate mapping of the resident macrophage progenitors.
FAQs
Erythropoietin and cooperating growth signals support the progenitors as they proliferate and move toward erythroid maturation. Their combined activity allows one early progenitor to produce multiple rounds of descendant cells rather than forming only a small cellular group. Observing the resulting colony therefore connects external signaling conditions with both expansion and progression toward erythroblast production.
The burst-forming pattern reflects the capacity of an early erythroid progenitor to expand extensively before its descendants mature. Large multicellular colonies provide evidence of repeated proliferation linked to erythroid development, whereas later erythroblast production indicates progression along the lineage. This combination helps distinguish progenitor potential from maturation alone in developmental studies.
Three measurements are especially informative: progenitor abundance, colony-forming capacity, and progression toward erythroblast production. Abundance reflects how many progenitors are present, colony formation reflects their ability to generate descendants, and maturation reflects developmental advancement. Examining these features together can reveal whether a change affects progenitor numbers, expansion, differentiation, or several stages at once.
A typical assay places hematopoietic progenitors in semisolid culture containing erythropoietin and cooperating growth signals. Individual progenitors then proliferate within the matrix, producing colonies that can be evaluated for size or abundance and followed as they mature toward erythroblasts. The culture format links each observable colony to the behavior of an early erythroid progenitor.
Researchers can examine progenitor abundance and colony-forming behavior in samples derived from different tissues or developmental stages. Comparing the resulting colonies indicates whether the sources contain different numbers of early erythroid progenitors or differ in their capacity for expansion and maturation. This makes the assay useful for mapping changes in blood formation during development.
BFU-E assays provide measurable erythropoietic outcomes after a genetic change, signaling manipulation, or external exposure. Differences in progenitor abundance, colony formation, colony expansion, or erythroblast progression can show which part of erythroid development is affected. In developmental biology, this connects molecular or environmental influences with the cellular stages that produce red blood cells.