Intracerebroventricular Injection
Intracerebroventricular Injection - Related Videos
08:01
Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
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Cited by 136 •
2016
The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.
05:29
Free-Hand Intracerebroventricular Injections in Mice
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Cited by 7 •
2024
Here, a simple and rapid approach for performing intracerebroventricular injections in mice using a free-hand approach (that is, without a stereotaxic device) is described.
08:27
Establishment of a Valuable Mimic of Alzheimer's Disease in Rat Animal Model by Intracerebroventricular Injection of Composited Amyloid Beta Protein
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Cited by 15 •
2018
This is a protocol to mimic Alzheimer's Disease in rats by evaluation of spatial memory impairment, neuronal pathological changes, neuronal amyloid beta protein (Aβ) burden, and neurofibrillary tangles aggregation, induced by the injection of Aβ25-35 combined with aluminum trichloride and recombinant human transforming growth factor-β1.
03:24
Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain
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2023
This video demonstrates ommaya mediated intracerebroventricular injection in a mouse model. The method enables localized delivery of potential drug candidates and evades the blood-brain barrier.
05:55
Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice
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Cited by 122 •
2011
This article demonstrates two very different methods of injection: 1) into the brain (intracerebroventricular) and 2) systemic (intravenous) to introduce the therapeutic agents into the central nervous system of neonatal mice.
FAQs
The ventricular route changes exposure by placing the compound in cerebrospinal fluid rather than relying only on systemic delivery. Because cerebrospinal fluid circulates through the ventricular system, the agent can disperse within that fluid and partly bypass the blood-brain barrier. This makes the approach useful when a compound’s central nervous system access is limited after administration through the body.
Accurate anatomical placement is central to the method’s performance. If the needle or catheter is not positioned within the intended cerebral ventricle, delivery may not occur as planned, and tissue injury becomes a concern. Controlled dosing is equally important because the procedure can alter intracranial pressure or produce other procedure-related effects that complicate interpretation.
Intracerebroventricular injection differs from systemic administration primarily in the route used to reach the brain. Systemic delivery must depend on distribution from the circulation, whereas ventricular administration places the substance in cerebrospinal fluid and can partly circumvent the blood-brain barrier. This comparison is especially relevant when evaluating agents intended to act within the central nervous system.
At a procedural level, the intervention requires positioning a needle or catheter within a cerebral ventricle and delivering a controlled amount of the selected drug, biological agent, or experimental compound. The workflow also includes careful assessment for tissue injury, altered intracranial pressure, and other procedure-related effects, since these outcomes can influence both safety and experimental conclusions.
Researchers may choose this route for central nervous system pharmacology, studies of neurological disease mechanisms, and investigations of treatments that do not readily reach brain tissue through systemic administration. By placing the test substance in ventricular cerebrospinal fluid, the approach helps examine central effects while reducing reliance on passage from the bloodstream.
In medicine, the method can support evaluation of a compound’s effects when delivered near the brain’s fluid spaces, but results require cautious interpretation. Observed effects may reflect the intended agent, the delivery route, or complications such as tissue injury and pressure changes. Researchers therefore relate outcome measurements to placement accuracy, dose control, and procedure-related assessments.