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Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
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Chapters
- 00:05Title
- 01:30Aβ Peptide and Syringe Preparation
- 02:54Aβ Injection in Mouse
- 06:06Results: Confirmation of Prepared Aβ Species via SDS-PAGE and Y-maze Behavioral Tests
- 07:07Conclusion
The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.
