Hek 293t Cells
Hek 293t Cells - Related Videos
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Cited by 23 •
2011
Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).
09:29
Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
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Cited by 1 •
2024
This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.
09:33
Rapid Isolation of the Mitoribosome from HEK Cells
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Cited by 25 •
2018
Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
09:02
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Cited by 1 •
2020
This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
FAQs
The SV40 large T antigen enables replication of plasmids that contain an SV40 origin. This plasmid replication can increase the amount of template available for transgene expression, helping explain why constructs with the appropriate origin can produce strong signals after transfection. The effect is therefore tied to plasmid design, not simply cell growth.
An SV40 origin provides the sequence recognized in the large-T-antigen context of these cells. Without that matching feature, the specific replication-based advantage described for HEK 293T cells would not apply in the same way. Researchers therefore consider both the cell line and plasmid architecture when aiming to enhance transgene expression.
Two properties work together: robust growth supports the expansion of cell material, while high transfection efficiency allows genetic constructs to enter many cells effectively. This combination shortens the path from construct introduction to measurable gene expression, making the line useful for screening, assay development, and experiments that require rapid genetic manipulation.
Expression depends on more than introducing a construct. In HEK 293T cells, the SV40 large T antigen can promote replication when the plasmid carries an SV40 origin, which may increase transgene expression. This distinction helps researchers interpret results according to construct features rather than treating all transfected plasmids as equivalent.
A typical high-level workflow begins with growing the cells, introducing a genetic construct by transfection, and allowing the cells to express the encoded product. Researchers then use the resulting system for recombinant protein or viral-vector production, depending on the construct and experimental goal. The overview supports this workflow without specifying a single universal protocol.
Experiments that require quick testing of genetic constructs can benefit from this feature. Examples supported by the overview include gene-function studies, screening, and assay development. Because constructs can be introduced efficiently and expression can be enhanced under the appropriate plasmid conditions, researchers can use the cells for rapid evaluation of molecular effects.
They connect construct-level questions with cell-based outcomes. Researchers can introduce genetic material to examine gene function, while the same expression capacity supports production of recombinant proteins or viral vectors. This versatility makes the line useful across molecular and cell biology, from testing how a gene behaves to generating materials needed for further experiments.