Immune Cell Enrichment
Immune Cell Enrichment - Related Videos
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
13:58
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
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Cited by 28 •
2012
This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
06:32
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
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Cited by 28 •
2010
This protocol describes the isolation, enrichment, and maintenance of medulloblastoma tumor stem cells derived from mutant mice with ectopic Sonic hedgehog pathway activity.
08:32
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Cited by 9 •
2018
Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.
FAQs
Selection depends on the feature that best distinguishes the desired population from surrounding cells. Cell-surface markers support marker-based separation, whereas physical properties or density can guide flow-based separation or density-gradient centrifugation. This choice determines which population can be concentrated, such as T cells, B cells, natural killer cells, or tumor-infiltrating lymphocytes, for a more focused cancer analysis.
Magnetic-activated cell sorting, flow-based separation, and density-gradient centrifugation use different selection bases rather than representing interchangeable versions of one mechanism. Magnetic sorting and flow-based approaches can use cellular characteristics, while density gradients separate according to density. Researchers therefore match the approach to the distinguishing features present in the sample and the immune population they need to study.
Blood and tumor tissue provide different experimental contexts for enriched immune populations. Blood can provide immune cells present in the circulation, while tumor tissue can provide tumor-infiltrating lymphocytes associated directly with the tumor immune microenvironment. Choosing the source therefore affects whether the analysis emphasizes immune populations in blood or cells located within the tumor environment.
A practical workflow begins by identifying the immune population and selecting a separation basis, such as a cell-surface marker, physical property, or density difference. Researchers then apply a compatible approach, including magnetic-activated cell sorting, flow-based separation, or density-gradient centrifugation, to the biological sample. The resulting enriched cells can be directed to immune-function or cancer-focused analyses.
Enriched immune populations allow researchers to examine selected cellular components of the tumor immune microenvironment rather than relying only on a complex mixed sample. T cells, B cells, natural killer cells, and tumor-infiltrating lymphocytes can be studied as distinct populations. This supports more focused characterization of immune involvement within cancer research.
Researchers use enriched cells to measure immune responses to treatment and to investigate features relevant to cancer immunotherapies. The same populations can contribute to biomarker development by providing more focused material for analysis. These applications connect cell isolation with questions about treatment response, immune function, and the identification of measurable indicators in cancer research.