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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

DOI:
10.3791/4420-v

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13:58 min

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October 22, 2012

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1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit,Massachusetts General Hospital and Harvard Medical School

Chapters

  • 00:05Title
  • 01:50Cell Isolation from Lymphoid Tissue
  • 03:35Tetramer Staining
  • 04:18Magnetic Enrichment
  • 06:39Flow Cytometry
  • 09:07Results: Calculation of MHC Tetramer-based Enrichment of Epitope-specific T Cells
  • 13:15Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.

Tags

Peptide:MHC Tetramer-based EnrichmentEpitope-specific T CellsT Cell Antigen Receptor SpecificityFunctional ResponseProliferationCytokine ProductionActivation MarkersTCR Transgenic Adoptive Transfer ModelMonoclonal T CellsHistocompatible HostsCongenic Marker AntibodyExperimental ArtifactsFunctional HeterogeneityPolyclonal Population

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