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JoVE Journal
Immunology and Infection

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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Article DOI: 10.3791/4420
October 22nd, 2012


Summary October 22nd, 2012

This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.

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