Radiosensitivity Assay
Radiosensitivity Assay - Related Videos
04:53
Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors
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2022
The zebrafish has recently been exploited as a model to validate potential radiation modifiers. The present protocol describes the detailed steps to use zebrafish embryos for radiation-based screening experiments and some observational approaches to evaluate the effect of different treatments and radiation.
08:32
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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2025
A meticulous and structured approach is given to select resistant and sensitive genes of radiation through the application of a genome-wide CRISPR/Cas9 screen method. This protocol also has the potential to serve as a versatile framework for other research endeavors investigating the mechanisms of resistance to clinically administered chemical drugs.
00:09
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
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2019
The presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell lines.
05:39
Live Imaging to Quantify Cellular Radiosensitivity in Patient-Derived Tumor Organoids
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Cited by 1 •
2024
Here, we detail a straightforward live imaging approach for quantifying the sensitivity of patient-derived tumor organoids to ionizing radiation.
08:12
The TUNEL Assay
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2023
One of the hallmarks of apoptosis is the nuclear DNA fragmentation by nucleases. These enzymes are activated by caspases, the family of proteins that execute the cell death program. TUNEL assay is a method that takes advantage of this feature to detect apoptotic cells. In this assay, an enzyme called terminal deoxynucleotidyl transferase catalyzes the addition of dUTP nucleotides to the free 3’ ends of fragmented DNA. By using dUTPs that are labeled with chemical tags that can produce...
FAQs
Clonogenic survival focuses on whether an irradiated cell retains the capacity to keep dividing and form a surviving population. This makes it more informative than measuring immediate cell viability alone when the research question concerns durable radiation response. In cancer studies, it helps distinguish cells that recover from radiation-induced DNA damage from those that lose reproductive potential.
Dose-response data show how survival changes as the radiation dose increases. A phenotype with greater loss of surviving cells across defined doses can be characterized as more radiosensitive, whereas persistence of survival indicates greater radioresistance. Comparing these patterns helps investigators characterize variation among tumor cells and relate that variation to cancer treatment-response research.
The assay can support studies of DNA repair and cell-death pathways that shape the response to ionizing radiation. Surviving-cell measurements provide an outcome through which investigators can examine whether cells repair radiation-induced damage and continue dividing or instead lose their ability to reproduce. This links measurable survival patterns with biological mechanisms relevant to cancer research.
Investigators begin with cultured tumor cells and expose them to defined radiation doses. They then quantify the cells that survive, often by assessing clonogenic survival, and analyze the resulting dose-response data. This workflow produces a comparative measure of radiation response while allowing researchers to characterize radiosensitive or radioresistant phenotypes in a controlled laboratory setting.
These assays provide a way to test whether a drug changes the response of tumor cells to radiation. Investigators can assess the resulting survival pattern and determine whether the treatment is associated with greater radiation sensitivity. The approach supports research on drug strategies intended to influence radiation response and helps connect cellular outcomes with cancer treatment development.
By revealing differences in radiation response among tumor cells, the assay contributes to treatment-response research aimed at understanding which cancer phenotypes are more sensitive or resistant. Its dose-response results can inform investigations of individualized radiotherapy strategies, particularly when researchers are evaluating how tumor biology and potential radiosensitizing treatments may affect radiation-related outcomes.