Endometrial Epithelial Cells
Endometrial Epithelial Cells - Related Videos
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 77 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
09:15
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
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Cited by 15 •
2014
Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human...
05:12
Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells
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Cited by 3 •
2024
Here, we present a protocol to isolate and culture rat endometrial epithelial stem cells (reESCs), generating rat endometrial organoids. This method facilitates in vitro studies of endometrial diseases, enabling gene editing and other cellular manipulations.
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
FAQs
The two ovarian hormones support complementary phases of epithelial behavior. Estrogen promotes cell proliferation, increasing renewal of the lining, whereas progesterone encourages differentiation and secretory activity. Studying this transition helps explain how the tissue changes from growth to a state that can support embryo implantation, rather than treating hormone response as a single effect.
Remodeling allows the endometrial lining to remain responsive rather than static. In developmental biology, endometrial epithelial cells can therefore be examined for coordinated renewal, hormone-dependent changes, and signaling within a changing tissue environment. These properties make them useful for investigating how maternal tissues prepare for and interact with an embryo during reproductive events.
Their position in the uterine lining places them at the interface between maternal tissue and the developing embryo. Researchers can study how epithelial behavior, secretory functions, and tissue remodeling relate to implantation. This perspective also connects cellular responses with broader questions in reproductive tract development, including how specialized epithelial tissues are maintained and prepared.
Primary cultures, organoids, and engineered endometrial tissues provide complementary experimental platforms. These systems support examination of epithelial behavior outside the intact uterus while retaining a focus on tissue preparation and embryo-related processes. Together, they allow investigators to study renewal, signaling, remodeling, and hormone-associated functions in defined research settings.
Experiments can connect hormone-responsive epithelial changes with the secretory functions and tissue remodeling associated with implantation. They may also clarify how the maternal lining interacts with a developing embryo. In developmental biology, these observations help relate cellular behavior to tissue-level preparation, rather than examining implantation only as an organism-level event.
These models are used to investigate implantation, infertility, and menstrual disorders, while also supporting research on reproductive tract development. Their value comes from linking clinically relevant outcomes with cellular processes such as epithelial renewal, signaling, remodeling, and hormone-dependent differentiation. This makes them relevant to both developmental biology and reproductive health research.