Genomic Pcr
Genomic Pcr - Related Videos
02:44
Single Worm PCR: A Method to Extract and Amplify Genomic DNA
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2023
This video describes a technique to rapidly screen a single nematode for a genetic marker by PCR screening. In the example protocol, we screen for CRISPR-based genome editing.
14:07
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
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Cited by 9 •
2012
An efficient genome-wide single gene mutation method has been established using Streptococcus sanguinis as a model organism. This method has achieved via high throughput recombinant PCRs and transformations.
03:20
Nested-PCR to Detect a Specific Viral Genomic Sequence
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2025
This video describes nested polymerase chain reaction, a technique that consists of two sequential PCR amplification processes using two primer sets. The first set of primers is intended to anneal to sequences upstream of the second set, resulting in selective amplification of specific gene sequences. This PCR is more sensitive and specific than a normal PCR and is widely used as a detection technique for various diseases.
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
FAQs
Flanking primers bind to complementary sequences on opposite sides of the region of interest. Their positions define the boundaries of the DNA segment copied during extension, allowing a selected locus to be examined within a much larger genome. This targeting is especially important when researchers need to distinguish a specific genetic change from unrelated genomic sequences.
Each cycle separates the two DNA strands, provides conditions for primers to anneal to their complementary target sites, and allows a thermostable DNA polymerase to extend the primers. Repeating these coordinated steps generates additional copies of the selected region, progressively increasing the amount of locus-specific DNA available for analysis.
The amplified region reflects the sequence located between the primer-binding sites. If an insertion or deletion changes that region, the resulting PCR product can reveal that the genomic locus differs from the expected arrangement. This makes the method useful for checking genetic modifications and distinguishing altered genotypes in developmental biology experiments.
Genomic PCR supplies genetic evidence that can be compared with an observed developmental trait. By verifying whether embryos, cells, or model organisms carry a particular allele, insertion, or deletion, researchers can relate inherited or engineered DNA changes to developmental outcomes. This connection supports investigations of gene function and molecular regulation during development.
A typical analysis begins with genomic DNA containing the locus of interest, followed by selection of primers that flank the target region. The DNA then undergoes repeated denaturation, primer annealing, and polymerase extension under thermal cycling conditions. The resulting amplified region is examined to determine whether the expected genetic sequence or alteration is present.
They would use Genomic PCR when a visible phenotype needs genetic verification or when a developmental change may not be apparent from observation alone. The assay can confirm genotypes, detect inserted or deleted sequences, and establish whether embryos, cells, or model organisms carry the genetic change being studied, strengthening interpretation of developmental experiments.