Chimeric Mouse Generation
Chimeric Mouse Generation - Related Videos
21:01
Generating Chimeric Zebrafish Embryos by Transplantation
0 Views •
Cited by 61 •
2009
A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.
11:25
Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
0 Views •
Cited by 33 •
2015
We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.
04:42
An Ex Vivo Technique to Generate Tumor-Specific Chimeric Antigen Receptor T Cells
0 Views •
2025
This video demonstrates a technique for the ex vivo generation of tumor antigen-specific chimeric antigen receptor (CAR) T cells. Retroviral vectors carrying transgenic RNA are mixed with spleen cells in a medium supplemented with interleukin-2 to promote T-cell survival and proliferation. The mixture is then transferred to a plate coated with recombinant human fibronectin fragments and centrifuged to facilitate the fusion of the virus with the cell, allowing the RNA to integrate into the host...
10:29
Generation of Human Chimeric Antigen Receptor Regulatory T Cells
0 Views •
Cited by 5 •
2025
This protocol provides a streamlined workflow to generate and test human chimeric antigen receptor regulatory T cells (CAR Tregs).
07:17
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
0 Views •
Cited by 1 •
2020
This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.
FAQs
After hematopoietic stem and progenitor cells enter an immunodeficient recipient, they can engraft within the living host, proliferate, and generate immune cell populations. This sequence creates an experimental system in which investigators can examine immune development and function after the introduced cells establish themselves in the recipient environment.
The approach places genetically distinct cells or tissues within a shared living setting. Differences that remain associated with the introduced cells can indicate cell-intrinsic influences, whereas effects shaped by the recipient setting point to environmental contributions. This distinction helps clarify whether an immune phenotype arises from the cells themselves or their surroundings.
The selected source determines which biological contribution the model is designed to examine. Hematopoietic stem and progenitor cells support immune reconstitution, while embryonic cell contribution or tissue transplantation provides alternative ways to study genetically distinct cellular or tissue effects. Matching the source to the research question allows more focused analysis of immune biology.
When appropriately sourced human cells are introduced into a suitable chimeric model, the resulting system can include human immune components within a mouse setting. This design provides a platform for investigating immune function, infection, and therapeutic strategies with greater relevance to human immune biology than a system containing only mouse-derived components.
A common workflow begins with an immunodeficient recipient and the introduction of hematopoietic stem and progenitor cells from a genetically distinct source. Investigators then assess whether the cells engraft, proliferate, and reconstitute immune populations. The resulting mouse can be used for controlled studies of immune development, responses, or infection.
Researchers can use these mice when they need to examine pathogen infection together with the host immune response in a controlled living system. The model permits investigation of how introduced immune components participate in infection-related processes, while the chimeric design helps distinguish contributions from immune cells and the surrounding host environment.
These models can support analysis of immune development, immune function, pathogen infection, and host responses. They also provide platforms for evaluating therapeutic strategies. Because the mouse contains contributions from genetically distinct sources, investigators can connect observed outcomes to particular immune components or to the recipient environment, strengthening interpretation of experimental results.