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JoVE Journal
Genetics

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Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
 

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Article DOI: 10.3791/60156-v
January 29th, 2020

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Summary January 29th, 2020

This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.

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