Monolayer Permeability
Monolayer Permeability - Related Videos
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
0 Views •
Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
14:18
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
0 Views •
Cited by 1 •
2011
Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).
03:28
A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins
0 Views •
2025
This video demonstrates a permeable membrane insert-based infection system to assess the impact of secreted bacterial toxins. By eliminating direct contact between the bacterial pathogen and the host cells using a permeable membrane, this technique allows for studying the effect of toxins secreted by pathogenic bacteria on mammalian host cells.
07:03
An in vivo Assay to Test Blood Vessel Permeability
0 Views •
Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
0 Views •
Cited by 3 •
2015
The modified Landis technique enables paired measurement of the hydraulic conductivity of individual microvessels in the mesentery of normal and genetically modified rats under control and test conditions using microperfusion techniques. It provides a convenient method to evaluate mechanisms that regulate microvessel permeability and transvascular exchange under physiological conditions.
FAQs
Confluence creates a continuous cellular layer, allowing transport across the cultured cells to be interpreted as a property of the barrier rather than simply an uncovered region of the membrane. If the layer is not continuous, tracer or compound movement may reflect gaps instead of altered barrier function, weakening conclusions about health, disease, or experimental treatment effects.
These experimental factors can change how effectively an epithelial or endothelial layer restricts movement between the two chambers. The assay captures that change through the amount of tracer or test compound appearing on the opposite side. Comparing conditions therefore helps connect immune activity or infection with altered barrier integrity in a controlled culture model.
Both cell-layer models can be examined through transport across a porous membrane, but they represent different barrier contexts. Epithelial systems support studies of tissue-facing protection, whereas endothelial systems provide a model for vascular barrier behavior. Applying the same permeability logic to either layer helps investigators examine how inflammation, immune-cell interactions, or pathogens affect distinct tissue interfaces.
Cells are cultured until they form a confluent layer on a porous membrane that separates two chambers. A tracer or test compound is then introduced on one side, followed by measurement of its appearance on the other side. The resulting transport measurement is used to estimate how intact the cellular barrier remains under the selected experimental condition.
The detected tracer provides a measurable readout of movement through the cultured barrier system. Its appearance on the receiving side can be quantified and interpreted as evidence of transport across the cell layer. When experimental conditions change that measurement, the result helps assess whether barrier integrity or permeability has been altered.
This approach is useful when researchers need to examine how pathogens, inflammatory mediators, or interactions with immune cells affect epithelial or endothelial barriers. It can support investigations of infection mechanisms and tissue protection, while also providing an experimental basis for evaluating candidate therapies intended to preserve or modify barrier function.