Rat Kidney Injury
Rat Kidney Injury - Related Videos
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Cited by 141 •
2013
We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.
07:29
Intravascular Delivery of Biologics to the Rat Kidney
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Cited by 1 •
2016
The administration of drugs for recovery of kidney function requires control of the localization and distribution of the therapeutic compound. Here, we describe in detail a simple technique for intrarenal delivery of drugs in rats. This procedure may be easily performed with no mortality and high reproducibility.
03:30
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
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2023
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
The initiating stress shapes the biological response being examined. Ischemia, nephrotoxic compounds, infection, and inflammation can each disrupt renal tissue through different pathways, while all may impair kidney function. Comparing these triggers helps researchers distinguish injury-specific effects from shared consequences, such as tubular damage, reduced filtration, oxidative stress, and inflammatory activation.
Tubular epithelial cells are a key structural target because their damage can interfere with normal kidney function and contribute to impaired filtration. Tissue injury in these cells may therefore connect microscopic structural changes with measurable functional outcomes. Examining both cellular damage and renal performance gives a more complete picture than relying on one type of result.
Oxidative stress and inflammatory responses are important molecular features of kidney damage, rather than merely secondary observations. They may accompany disruption caused by ischemia, toxic exposure, infection, or other insults. Measuring related molecular biomarkers can help investigators examine injury mechanisms and evaluate whether a potential protective therapy affects the underlying response as well as kidney function.
Assessment commonly combines functional, behavioral, structural, and molecular readouts. Serum creatinine provides an indicator of altered renal function, while urine production supplies another functional outcome. Histology reveals tissue-level changes, and molecular biomarkers help investigate injury pathways. Using these measures together allows researchers to relate kidney performance to structural damage and biological mechanisms.
A study generally begins by applying a defined source of renal stress, such as ischemia or a nephrotoxic compound, and then examining the resulting effects. Researchers assess urine production and serum creatinine, inspect kidney tissue through histology, and analyze molecular biomarkers. This combination supports comparison of functional impairment, structural injury, and mechanistic responses.
These models are useful when investigators need to study acute kidney injury, chronic renal disease, or nephrotoxicity in a controlled experimental setting. They can reveal how damaging exposures affect renal structure and function, provide preclinical evidence for protective therapies, and support evaluation of diagnostic approaches. Their value lies in connecting disease mechanisms with measurable outcomes.