Respirometric Profiling
Respirometric Profiling - Related Videos
08:11
Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays
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Cited by 2 •
2022
Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.
11:10
Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
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Cited by 32 •
2011
Saponin-permeabilized fiber preparation in conjunction with respirometric oxidative phosphorylation analysis provides integrative assessment of mitochondrial function. Mitochondrial respiration in physiological and pathological states can reflect various regulatory influences including mitochondrial interactions, morphology and biochemistry.
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Cited by 24 •
2015
Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
08:03
Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
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Cited by 1 •
2025
This study introduces a methodology for studying xenobiotic effects on mitochondrial function via respirometric and enzymatic assays. It examines electron transport chain modulation in various species, offering insights for targeted biopesticide development against insect vector-borne diseases.
FAQs
Time-resolved gas measurements turn respiration into a kinetic profile rather than a single endpoint. As oxygen concentration falls or carbon dioxide rises, the recorded change over time indicates how actively the biological sample is respiring under the selected conditions. Holding those conditions constant allows profiles from different samples or treatments to be compared quantitatively.
Choosing oxygen, carbon dioxide, or both depends on which gas change best captures the experimental question. Oxygen consumption reports disappearance of oxygen from the system, whereas carbon dioxide production reports its accumulation. Measuring both can provide a broader description of respiratory behavior, while using one can simplify monitoring when the other signal is not required.
Substrates, inhibitors, and other treatments reveal respiratory responses by shifting the measured gas trajectory or rate. A nutrient can be evaluated for its effect on respiration, while an inhibitor can expose sensitivity of the process to metabolic disruption. Comparing treated and untreated profiles helps distinguish a baseline rate from a response caused by the experimental condition.
Respirometric profiling contributes functional evidence about mitochondrial function and cellular bioenergetics, meaning how cells generate and use energy. It adds information about the integrated respiratory outcome that complements molecular and biochemical analyses. Using these approaches together can connect altered respiration with other findings in disease-focused studies without relying on a single type of measurement.
A typical workflow places cells, tissues, or microorganisms under controlled conditions and follows oxygen consumption, carbon dioxide production, or both over time with sensors. Researchers then compare respiration rates and examine changes after exposure to nutrients, inhibitors, or other treatments. The resulting profile links a defined condition to a measurable metabolic response.
The method can examine cells, tissues, or microorganisms, allowing investigators to select the system relevant to their question. This breadth supports measurements of respiration across different biological contexts while retaining a common readout: gas change over time. Comparing rates within the chosen system can show how substrates, inhibitors, or other treatments alter its metabolic behavior.
An altered rate or gas trajectory indicates that the tested condition changed the sample’s respiratory behavior, but the result is most informative when compared with another sample measured under the same controlled conditions. In medicine, these comparisons can support assessment of mitochondrial dysfunction, disease-related metabolic changes, toxic effects, or responses to therapeutic treatment.