Cell Preparation
Cell Preparation - Related Videos
09:39
Preparation of Drosophila S2 cells for Light Microscopy
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Cited by 24 •
2010
Drosophila Schneider (S2) cells are an increasingly popular system for the discovery and functional analysis of genes. Our goal is to describe some of the microscopic techniques that make S2 cells such an increasingly important experimental system.
07:42
Chromosome Preparation From Cultured Cells
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Cited by 146 •
2014
Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).
03:01
Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling
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2023
This video describes a technique of preparing breast cancer cell samples for metabolite profiling. Breast cancer cells are treated with estradiol, which binds to the estrogen receptor alpha and causes change in the metabolite...
03:38
Preparation of a Microfluidic Plate with Bacterial Cells
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2025
Source: Sutlief, A. L., et al. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (2019).This video demonstrates the preparation of a microfluidic plate for shear stress studies in bacterial cultures. After priming the channels with media, bacterial cells are introduced into the experimental channel and incubated for attachment. Residual media and culture are removed to prepare the system for subsequent shear...
02:30
Preparation of a Single-Cell Bacterial Inoculum
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2025
Source: Niu, L., et al., Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy. J. Vis. Exp. (2019)This video demonstrates the preparation of a Mycobacterium marinum single-cell suspension from a late-log phase culture for consistent use in infection assays and downstream applications.
FAQs
These conditions help preserve cell viability and phenotype during preparation. Excessive delays, unsuitable temperatures, or harsh handling can reduce the quality of the starting material, making later measurements less reliable. Controlling these variables is especially important when experiments examine neuronal structure, signaling, gene expression, or responses to drugs and injury.
Dissociation separates tissue or cultured material into individual cells, creating a more uniform starting format for plating or analysis. This organization supports measurements that depend on examining cells separately, including neuronal morphology, cellular responses, and other assays performed on defined cell populations. The process must be handled carefully so cell viability and phenotype remain suitable for experimentation.
Washing removes debris that could interfere with plating or analysis, while counting determines how much cellular material is available. Adjusting the suspension to a defined concentration makes the starting conditions more consistent between experiments. Together, these steps support comparable cultures and measurements by reducing variation in the number and cleanliness of cells entering an assay.
A typical workflow begins with isolating tissue or handling cultured cells, followed by dissociation when individual cells are needed. The material can then be washed to remove debris, counted, and adjusted to a defined concentration before plating or analysis. Throughout the workflow, researchers control temperature, timing, and handling to maintain suitable viability and phenotype.
The method is useful whenever investigators need consistent cellular material for studying nervous system function. Prepared cells can support analyses of neuronal structure, signaling, gene expression, and responses to drugs or injury. They may also serve as starting material for cultured experiments, imaging assays, electrophysiology experiments, and other studies requiring comparable samples.
Standardizing the prepared cell population reduces differences that originate before the experiment begins. Comparable cell numbers, cleanliness, viability, and phenotype make it easier to interpret changes measured by imaging, electrophysiology, molecular analysis, or drug-response studies. This consistency strengthens reproducibility and enables more meaningful comparisons among cultures, experimental conditions, and investigations of nervous system function.