Cardiac Cell Isolation
Cardiac Cell Isolation - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
0 Views •
Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
0 Views •
Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
12:15
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
0 Views •
Cited by 35 •
2009
Adult cardiac myocytes are primary cells that can be isolated from animal hearts and cultured for several days. Within this culture period adenoviral gene transfer can be used to express genetically encoded biosensors (GEBs) or fluorescent fusion proteins. Both approaches allow cellular investigations by means of confocal microscopy.
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
0 Views •
Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
0 Views •
Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
FAQs
Enzymatic digestion helps break down the extracellular matrix that holds heart tissue together. This loosens the tissue sufficiently for individual cardiac cells to be released while supporting preservation of cardiomyocytes and other cardiac cell populations. Because the method combines chemical tissue breakdown with controlled handling, researchers can obtain cells suitable for later characterization, culture, or functional analysis.
Gentle mechanical dissociation complements enzymatic digestion by helping separate cells after the extracellular matrix has been loosened. Limiting the mechanical force is important because the goal is to release cells while preserving their viability and functional properties. This combined approach supports downstream studies of cardiac structure, contraction, electrical signaling, and cellular responses.
The usefulness of an isolated cardiac-cell preparation depends on preserving viable cells and retaining relevant cardiac populations during tissue preparation, enzymatic digestion, and mechanical separation. A preparation can then be examined through characterization, culture, imaging, electrophysiology, or molecular assays. These downstream options allow researchers to connect cellular condition with structural, functional, or molecular outcomes.
A typical workflow begins with preparation of heart tissue, followed by enzymatic digestion to weaken the extracellular matrix. Gentle mechanical dissociation then helps separate the cells. The resulting cardiac cell populations may be characterized, placed in culture, or directed into imaging, electrophysiological, or molecular analyses. Each stage supports examination under controlled laboratory conditions.
Once cells have been isolated, researchers can assess their structure and function using several complementary approaches. Imaging can reveal cellular features, electrophysiology can examine electrical behavior, and molecular assays can evaluate molecular characteristics or responses. Cells may also be characterized or cultured before analysis, allowing experiments to address different aspects of cardiac biology.
In medicine, cardiac cell isolation provides a cellular model for investigating contraction, electrical signaling, and responses to cardiac injury. It also supports drug-safety studies and evaluation of regenerative strategies. By studying cells under controlled conditions, researchers can examine mechanisms that may be difficult to distinguish within intact heart tissue and assess potential therapeutic approaches.
Isolated cardiac cells help clarify disease mechanisms by allowing researchers to study cardiac behavior and cellular responses directly. The same preparation can support evaluation of drug effects, injury-related changes, and regenerative strategies. Findings from structural, electrophysiological, imaging, or molecular analyses can therefore contribute to understanding cardiac pathology and assessing possible treatments.