Cardiomyocyte Electrophysiology
Cardiomyocyte Electrophysiology - Related Videos
11:13
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
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Cited by 44 •
2017
Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
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Cited by 3 •
2011
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
11:33
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
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Cited by 15 •
2013
Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.
10:46
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
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Cited by 4 •
2015
Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
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Cited by 25 •
2016
Quantification of cardiomyocyte turnover is challenging. The protocol described here makes an important contribution to this challenge by enabling accurate and sensitive quantification of neo-cardiomyocyte nuclei generation and nuclei ploidy.
FAQs
Their controlled movement across the cell membrane creates the electrical changes underlying cardiomyocyte activity. Voltage-gated channels regulate when these ions enter or leave the cell, allowing the membrane voltage to change and produce an action potential. Calcium movement also connects this electrical event with contraction, while the combined ion movements influence signal transmission and electrical stability.
Voltage-gated channels provide the controlled pathway through which sodium, calcium, and potassium cross the membrane. Their activity shapes the action potential and determines how electrical signals develop and propagate through cardiac muscle. Because these signals regulate excitation–contraction coupling, altered channel behavior can affect both the electrical pattern and the coordinated contraction of heart muscle cells.
Electrical activity regulates excitation–contraction coupling, the process that links a membrane signal to mechanical contraction. Ion movement generates an action potential, and calcium is specifically involved in translating that electrical event into contraction. Studying this connection helps investigators relate abnormal membrane behavior to impaired coordination of cardiac muscle activity and rhythm-related dysfunction.
Membrane-voltage measurements show how electrical signals develop in a cardiomyocyte, whereas ion-current measurements examine the movement of charged particles through membrane channels. Together, these readouts can indicate whether cellular electrical behavior is altered. They provide mechanistic information that complements measurements of conduction and rhythm abnormalities in cardiac cells or tissues.
The experimental preparation determines which level of electrical behavior can be examined. Isolated cardiomyocytes allow investigators to assess cellular membrane voltage and ion currents, while cardiac tissues can support evaluation of signal conduction and coordinated rhythm behavior. Using either preparation helps match the measurement to the research question, from channel-level effects to tissue-level electrical abnormalities.
Researchers can use electrophysiological measurements to identify abnormal membrane voltage, ion currents, conduction, or rhythm patterns associated with arrhythmias. The same approach supports investigation of inherited channel disorders by examining how altered channel-related electrical behavior affects cardiomyocytes or cardiac tissues. These observations help connect cellular electrical changes with clinically relevant rhythm disturbances.
Drug-induced cardiotoxicity research uses electrical measurements to determine whether a treatment disrupts cardiomyocyte membrane behavior, ion currents, conduction, or rhythm. Detecting such changes provides evidence of electrical instability that may not be apparent from contraction alone. This information supports evaluation of drug effects and can guide the development of therapies intended to improve cardiac electrical stability.