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Immunology and Infection

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JoVE Journal Immunology and Infection
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Article DOI: 10.3791/2531-v • 11:11 min • December 27th, 2010
December 27th, 2010 •
1, 1, 1
1Laboratory Program, BC Centre for Excellence in HIV/AIDS

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HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.

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HIV-1 Tropism Genotypic Inference Population-based Sequencing V3 Region CCR5-antagonist Viral Population Coreceptor Tropism Testing Viral RNA Reverse Transcriptase-PCR Software Analysis RE Call Bioinformatic Algorithm Geno2pheno False Positive Rate
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