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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Article DOI: 10.3791/50883-v • 10:30 min • January 12th, 2014
January 12th, 2014 •
*1, *1, 1, 1
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon
* These authors contributed equally

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We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

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PHluorin Axon Guidance Receptors Cell Culture Chick Embryo Development Attractive Cues Repulsive Cues Signaling Mechanisms Growth Cones Ligands Cell Surface Dynamics Spatio-temporal Visualization In Vitro Validation In Vivo Assessment
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