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Journal / Immunology and Infection / Bioenergetics and the Oxidative Burst: Protocols…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bioenergetics and the Oxidative Burst
Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
Journal (Immunology and Infection)
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In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
Journal (Immunology and Infection)
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Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

Article DOI: 10.3791/51301-v • 11:40 min • March 27th, 2014
March 27th, 2014 •
*1, *1, 1, 1, 1, *1
1UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology, University of Alabama at Birmingham
* These authors contributed equally

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Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.

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Bioenergetics Oxidative Burst Isolation Evaluation Human Leukocytes Platelets Mitochondrial Dysfunction Pathological Conditions Atherosclerosis Diabetes Septic Shock Neurodegenerative Diseases Bioenergetic Function Patients Hyperglycemia Inflammation Circulating Leukocytes Platelets Magnetic Bead Isolation Techniques Cell Adhesion Techniques High Throughput Microplate Respirometry Extracellular Flux Analyzer
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