Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Konark Mukherjee; Helen R. Clark; Vrushali Chavan; Emily K. Benson; Grahame J. Kidd; Sarika Srivastava

doi:10.3791/54214

Journal

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Neurociência

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Análise do Cérebro mitocôndrias Usando Serial Block-cara Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Análise do Cérebro mitocôndrias Usando Serial Block-cara Scanning Electron Microscopy

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13,983 Views

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07:47 min

•

July 09, 2016

DOI:

10.3791/54214-v

DOI:

10.3791/54214-v

•

07:47 min

July 09, 2016

•

1 Views

Konark Mukherjee¹, Helen R. Clark¹, Vrushali Chavan¹, Emily K. Benson², Grahame J. Kidd², Sarika Srivastava¹

¹Virginia Tech Carilion Research Institute, ²Renovo Neural Incorporated

Transcrição

Summary

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.