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Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Article DOI: 10.3791/55048-v • 09:01 min • December 12th, 2016
December 12th, 2016 •
1, 1, 1
1Division of Biological Science and Technology, Yonsei University

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We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.

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Caenorhabditis Elegans Three-dimensional Cultivation Laboratory Nematodes Natural Conditions C Elegans Development Physiology Behavior Rotting Fruit 3D Environment Cultivation Technique Nematode Habitats Other Nematodes Tong Young Lee Graduate Student Otto Clave Cholesterol Solution Sterilization Lysogeny Broth Bacteria Culture Serial Dilution Sodium Chloride Solution
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