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JoVE Journal Genetics
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Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Article DOI: 10.3791/55130-v • 10:41 min • March 29th, 2017
March 29th, 2017 •
1, 1, 1
1NeoGenomics Laboratories

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Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

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Wild-type Blocking PCR Direct Sequencing Low-frequency Somatic Mutations Somatic Mutation Testing Myd88 Gene Bone Marrow Samples Highly Accurate Sensitive Information Neoplastic Cells Wild Type Blocking PCR Based Sequencing Assay Exon Five L265 Hotspot Forward And Reverse Primers Five Prime M13 Sequence Blocking Oligonucleotide Mutant Enrichment Target Specificity Oligo Tools Website
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