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An Aptamer-based Sensor for Unchelated Gadolinium(III)
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JoVE Journal Química
An Aptamer-based Sensor for Unchelated Gadolinium(III)

An Aptamer-based Sensor for Unchelated Gadolinium(III)

DOI:
10.3791/55216-v

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05:15 min

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January 09, 2017

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, , , ,
1Department of Chemistry and Biochemistry,California State University, East Bay

Capítulos

  • 00:05Título
  • 00:36Preparation of Aptamer Stock Solutions and 2x Gd-sensor Solution
  • 01:52Constructing the Fluorescence Calibration Curve and Detecting the Presence of Unchelated Gd3+ in a Solution of Gd Contrast Agent
  • 03:15Results: Changes in the Fluorescence Emission of the Aptamer-based Sensor in the Presence of Gadolinium(III)
  • 04:33Conclusion

Summary

Tadução automática

Tadução automática

The use of polydeoxynucleotide (44-mer aptamer) molecules for sensing unchelated gadolinium(III) ion in an aqueous solution is described. The presence of the ion is detected via an increase in the fluorescence emission of the sensor.

Tags

AptamerGadoliniumFluorescenceMRI Contrast AgentUnchelatedToxicSensorAssayMetal CationThermal CyclingSerial Dilution

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