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3D磁干细胞聚集和生物反应器为成熟软骨再生

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

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3D磁干细胞聚集和生物反应器为成熟软骨再生

DOI:

10.3791/55221-v

•

09:46 min

•

April 27, 2017

•

Aurore Van de Walle, Claire Wilhelm, Nathalie Luciani

¹Laboratoire Matière et Systèmes Complexes (MSC),UMR 7057 CNRS and University Paris Diderot

Capítulos

00:05Título
01:05Magnetic Device Construction and Stem Cell Labeling
02:45Magnetic Cell Seeding
04:59Chondrocytes Differentiation
07:24Results: Representative Cartilage Regeneration Analyses
08:28Conclusion

Summary

Tadução automática

从干细胞软骨需要微调的培养条件。在这里，我们提出了冷凝细胞磁性的方法，一个必不可少的步骤启动软骨。此外，我们显示，在生物反应器中动态成熟施加机械刺激，以在蜂窝结构和增强软骨细胞外基质的产生。

Tags

3D Magnetic Stem Cell Aggregation Cartilage Regeneration Mesenchymal Stromal Cells Cell Condensation Cellular Bioreactor Dynamic Maturation Muscle Repair Cardiac Repair Vascular Repair Micromagnet Device Scaffold Seeding Iron Oxide Nanoparticles Magnetic Cell Labeling Cell Aggregation Cell Culture Trypsin-EDTA Cell Pellet Cell Counting Glass Bottom Petri Dish

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