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The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Article DOI: 10.3791/56761-v • 07:39 min • June 16th, 2018
June 16th, 2018 •
1, 2, 1,2, 1, 1, 3, 1,2
1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3Trauma Office, Children's National Health System

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An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.

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Lung Colonization Assay Circulating Tumor Cells Visualization Cancer Metastasis Extravasated Cancer Cells Procedure Cheng-Han Yang PHD Student Tumor Cell Culture Trypsin-EDTA Single-cell Suspension Micro Centrifuge Tube Medium Supplemented With 20%FBS End-over-end Rotation Incubation Viable Cells Trypan Blue Exclusion Sterile PBS
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